Click to buy
Rat Heat Shock 70kDa Protein 5 (HSPA5) ELISA Kit
on Gentaurs online shop

Product details

Estimated Turnaround Time

6-11 Business days

Target Name

Heat Shock 70kDa Protein 5

Target's alterntive name

MIF2, BIP, GRP78, Immunoglobulin heavy chain-binding protein, Glucose Regulated Protein 78, Binding Immunoglobulin Protein, Endoplasmic reticulum lumenal Ca binding grp78

Species Reactivity

Rattus norvegicus (Rat)

Sample Type

Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids.

Detection Range

0.781-50 ng/mL

Sensitivity

0.32 ng/mL

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Detection Method

Colorimetric

Assay Time

3 hours

Application

For research use only. Not for diagnostic procedures.

Assay Type

Double-antibody Sandwich

Shipping Conditions

Ice packs

Storage Temperature

Short term: 4°C; Long term: see manual.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Shelf life

Use Rat Heat Shock 70kDa Protein 5 (HSPA5) ELISA Kit before 8 months

Specificity

This assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 5

Cross Activity

No significant cross-reactivity or interference between Heat Shock 70kDa Protein 5 and analogues was observed.

Test Principle

No significant cross-reactivity or interference between Heat Shock 70kDa Protein 5 and analogues was observed.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Research Area

Signal transduction, Tumor immunity, Infection immunity

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Latin name

Rattus norvegicus