96 Tests
EKU04696
868 EUR
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Rat Heat Shock Transcription Factor 1 (HSF1) ELISA Kit
on Gentaurs online shop
6-11 Business days
Heat Shock Transcription Factor 1
HSTF1, Heat shock factor protein 1
Rattus norvegicus (Rat)
Tissue homogenates, Cell lysates and other biological fluids.
0.156-10 ng/mL
0.058 ng/mL
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Transcription Factor 1 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Transcription Factor 1 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Colorimetric
3 hours
For research use only. Not for diagnostic procedures.
Double-antibody Sandwich
Ice packs
Short term: 4°C; Long term: see manual.
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Use Rat Heat Shock Transcription Factor 1 (HSF1) ELISA Kit before 8 months
This assay has high sensitivity and excellent specificity for detection of Heat Shock Transcription Factor 1
No significant cross-reactivity or interference between Heat Shock Transcription Factor 1 and analogues was observed.
No significant cross-reactivity or interference between Heat Shock Transcription Factor 1 and analogues was observed.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Signal transduction, Tumor immunity, Developmental science
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Rattus norvegicus
Click to
buy
Rat Heat Shock Transcription Factor 1 (HSF1) ELISA Kit
on Gentaurs online shop
Shock