Detection Type

Colormetric

Target Species

Rat

Target Synonym

HSPB1, CMT2F, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27

Test principle

This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP-27/HSPB1. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP-27/HSPB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP-27/HSPB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat HSP-27/HSPB1. You can calculate the concentration of Rat HSP-27/HSPB1 in the samples by comparing the OD of the samples to the standard curve.

Sensitivity

0.47ng/mL

Detection Range

0.78~50ng/mL

Target Name

HSP-27/HSPB1

UNIProt ID

P42930

Tested Sample Types

Serum, Plasma, Cell supernatant

Product Name

Rat HSP-27/HSPB1(Heat Shock Protein 27) EIA Kit

Type

Sandwich

Description

This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.

Properties

MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Latin name

Rattus norvegicus

HeatShock