Human HSPD1(Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit

Product information

  • Size

    96T

  • Catalog number

    ELK1613

  • Price

    608 EUR

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Human HSPD1(Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit
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Product details

Alternative Names

HSP60; GROEL; CPN60; HuCHA60; Heat Shock Protein 60; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

Assay Type

Sandwich

Sensitivity

0.58ng/mL

Standard

100ng/mL

Detection range

1.56-100ng/mL

Assay length

3h

Research Area

Signal transduction;Tumor immunity;

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 60kD Protein 1, Chaperonin (HSPD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 60kD Protein 1, Chaperonin (HSPD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 60kD Protein 1, Chaperonin (HSPD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 60kD Protein 1, Chaperonin (HSPD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Click to buy
Human HSPD1(Heat Shock 60kD Protein 1, Chaperonin) ELISA Kit
on Gentaurs online shop