96T
ELK1846
608 EUR
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Rat HSPB1(Heat Shock 27kDa Protein 1) ELISA Kit
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HSP27; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock Protein 27; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;
Sandwich
0.26ng/mL
50ng/mL
0.781-50ng/mL
3h
Signal transduction;Tumor immunity;Developmental science;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 27kDa Protein 1 (HSPB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 27kDa Protein 1 (HSPB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 27kDa Protein 1 (HSPB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 27kDa Protein 1 (HSPB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Rattus norvegicus
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Rat HSPB1(Heat Shock 27kDa Protein 1) ELISA Kit
on Gentaurs online shop
Shock