Gallus HSPb2(Heat Shock Protein Beta 2) ELISA Kit

Product information

  • Size

    96T

  • Catalog number

    ELK2431

  • Price

    738 EUR

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Gallus HSPb2(Heat Shock Protein Beta 2) ELISA Kit
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Product details

Alternative Names

HSP-B2; MKBP; HSP27; Heat Shock 27kDa Protein 2; DMPK-binding protein

Assay Type

Sandwich

Sensitivity

5.7pg/mL

Standard

1000pg/mL

Detection range

15.62-1000pg/mL

Assay length

3h

Research Area

Signal transduction;Tumor immunity;Developmental science;

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 2 (HSPβ2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein Beta 2 (HSPβ2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 2 (HSPβ2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein Beta 2 (HSPβ2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Click to buy
Gallus HSPb2(Heat Shock Protein Beta 2) ELISA Kit
on Gentaurs online shop