Rat HSPb6(Heat Shock Protein Beta 6) ELISA Kit

Product information

  • Size

    96T

  • Catalog number

    ELK4263

  • Price

    608 EUR

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Rat HSPb6(Heat Shock Protein Beta 6) ELISA Kit
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Product details

Alternative Names

HSP20; Heat Shock Protein 20 kD; Heat Shock Protein B6, Alpha Crystallin Related; Heat shock 20 kDa-like protein p20

Assay Type

Sandwich

Sensitivity

0.068ng/mL

Standard

10ng/mL

Detection range

0.156-10ng/mL

Assay length

3h

Research Area

Infection immunity;

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 6 (HSPβ6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein Beta 6 (HSPβ6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 6 (HSPβ6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein Beta 6 (HSPβ6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Latin name

Rattus norvegicus

Click to buy
Rat HSPb6(Heat Shock Protein Beta 6) ELISA Kit
on Gentaurs online shop