96T
ELK8002
738 EUR
Click to
buy
Cattle HSPA1A(Heat Shock 70kDa Protein 1A) ELISA Kit
on Gentaurs online shop
HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70
Sandwich
0.59ng/mL
100ng/mL
1.56-100ng/mL
3h
Signal transduction;Tumor immunity;Infection immunity;Developmental science;
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1A (HSPA1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 1A (HSPA1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 1A (HSPA1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70kDa Protein 1A (HSPA1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Click to
buy
Cattle HSPA1A(Heat Shock 70kDa Protein 1A) ELISA Kit
on Gentaurs online shop
Shock