Our specialists from Gentaur/Genprice advise to follow carefully the instructions for use and store the Wheat Gliadin Polyclonal Antibody, Biotin Conjugated in refrigerator and according to the label upon arrival.
Manufactured in United States. Designed for Research Use Only, not for human or animal consumption.
If you buy Antibodies supplied by Epigen they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.