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Rabbit Anti-Human HSF1 Polyclonal: Biotin
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Product details

Immunogen

Purified human HSF1 protein NM_005526.2

Antibody's target

Human HSF1

Antibody's full description

Rabbit Anti-Human HSF1 Polyclonal

Primary research fields

Cancer, Heat Shock, Cell Signaling, Epigenetics, Cardiovascular System, Heart

Antibody's category

Polyclonal Antibodies

Antibody's other name

Heat shock factor 1 Antibody, Heat shock factor protein 1 Antibody, Heat shock transcription factor 1 Antibody, HSF 1 Antibody, hsf1 Antibody, HSF1_HUMAN Antibody, HSTF 1 Antibody, HSTF1 Antibody

Verified applications

WB, ICC/IF

Raised in

Rabbit

Antibody's reactivity

Human, Mouse, Rat

Antibody's recommended dilutions for use

WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Antibody's purified from

Protein A Purified

Recommended buffer for storage

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Antibody's concentration

1 mg/ml

Antibody's specificity

Detects ~85kDa (unstressed cell lysates), and ~95kDa (heat shocked cell lysates).

Storage recommendations

-20°C

Shipping recommendations

Blue Ice or 4°C

Certificate of analysis

1 µg/ml of SPC-208 was sufficient for detection of HSF1 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.

Antibody in cell

Cytoplasm, Nucleus

Tissue specificity

See included datasheet or contact our support service.

Scientific context

HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

Bibliography

1. Cotto J.J., Fox S.G. and Morimoto R.I. (1997) J. Cell Science 110: 2925-2934. 2. Morano K.A. and Thiele D.J. (1999). Gene Expression 7 (6): 271-82. 3. Tanaka K.I., et al. (2007). JBC Papers Online Manuscript M704081200. 4. Morimoto R. I. (1998) Genes Dev 12: 3788-3796. 5. McMillan D. R., et al. (1998) J Bio Chem 273: 7523-7528. 6. Jolly C., Usson Y. and Morimoto R.I. (1999) Proc. Natl. Acad. Sci. USA 96 (12): 6769- 6774. 7. Fiorenza M.T., et al. (1995) Nucleic Acids Res. 23 (3):467-474. 8. Goodson M.L., Park-Sarge O.K. and Sarge K.D. (1995) Mol. Cell. Biol. 15(10): 5288-5293. 9. Rallu M., et al. (1997) Proc. Natl. Acad. Sci. USA 94(6): 2392-2397. 10. Sarge K.D., et al. (1994) Biol. Reprod. 50(6): 1334-1343. 11. Murphy S.P., Gorzowski J.J., Sarge K.D. and Phillips B. (1994) Mol. Cell. Biol. 14(8):5309-5317.

Released date

25/Sep/2013

NCBI number

NP_005517.1

Gene number

3297

Protein number

Q00613

PubMed number

21167840

Tested applications

Western Blot

Tested reactivity

Mouse

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Representative figure link

Representative figure legend

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSF1 Polyclonal Antibody (SPC-208). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HSF1 Polyclonal Antibody (SPC-208) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSF1 Polyclonal Antibody (SPC-208). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-HSF1 Polyclonal Antibody (SPC-208) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h. Rabbit Anti-HSF1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-208) | Rabbit Anti-HSF1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Heat Shocked HeLa Cells (SPC-208)

Warning information

Non-hazardous

Country of production

Canada

Total weight (kg)

1.4

Net weight (g)

0.1

Stock availabilit

In Stock

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Conjugation

Biotinylated

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus