Rat HSPA1A(Heat Shock 70kDa Protein 1A) ELISA Kit

Product information

  • Size

    96T

  • Catalog number

    ELK7102

  • Price

    608 EUR

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Rat HSPA1A(Heat Shock 70kDa Protein 1A) ELISA Kit
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Product details

Alternative Names

HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70

Assay Type

Sandwich

Sensitivity

0.59ng/mL

Standard

100ng/mL

Detection range

1.56-100ng/mL

Assay length

3h

Research Area

Signal transduction;Tumor immunity;Infection immunity;Developmental science;

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1A (HSPA1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 1A (HSPA1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 1A (HSPA1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70kDa Protein 1A (HSPA1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Latin name

Rattus norvegicus

Click to buy
Rat HSPA1A(Heat Shock 70kDa Protein 1A) ELISA Kit
on Gentaurs online shop