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HSP70, heat shock protein 70, chloroplastic
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Product details

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Lyophilized

Immunogen

KLH-conjugated synthetic peptide derived from higher plant chloroplastic HSP70, inluding Arabidopsis thaliana cpHSC70-1, At4g24280 and cpHSC70-2, At5g49910

Raised in

Rabbit

Clonality

Polyclonal

Clone

Polyclonal

Purification

Serum

How to reconstitute

For reconstitution add 200 µl of sterile water.

Storage condition

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Verified applications

western blot (WB), immunoprecipitation (IP)

Connected products

AS08 371 | anti-HSP70 | heat shock protein 70 cytoplasmicAS08 347 | anti-HSP70 | heat shock protein 70, mitochondrialcollection of antibodies to plant heat shock proteins

Recommended dilutions for use

1: 2000 (WB), 1: 100 (IP)

Molecular weight (expected | аpparent)

76 | 70 kDa

Verified reactivity

Arabidopsis thaliana, Hordeum spontaneum, Oryza sativa, Pinus strobus, Pisum sativum

Possible reactivity

Sorghum bicolor, Arundo donax, Brassica rapa subsp. pekinensis, Brassica napus, Coffea canephora, Theobroma cacao, Brachypodium distachyon, Prunus persica, Ricinus communis, Phaseolus vulgaris, Solanum tuberosum, Solanum lycopersicum, Citrus sinensis, Citrus clementina.dicots including: Spinacia oleracea, Vitis vinifera, Capsella rubella, Medicago trancatula, Glycine max, Glycine soja monocots including: Oryza sativa, Zea mays, Hordeum Vulgare, Triticum aestivum trees: Picea sitchemsis, Populus trichocarpa moss: Physomitrella patens

No reactivity

no confirmed exceptions from predicted reactivity known in the moment

Supplementary information

To be added when available

References

Shen et al. (2016). The Arabidopsis polyamine transporter LHR1/PUT3 modulates heat responsive gene expression by enhancing mRNA stability. Plant J. 2016 Aug 19. doi: 10.1111/tpj.13310. [Epub ahead of print] Jedmowski et al. (2014). Comparative analysis of drought stress effects on photosynthesis of Eurasian and North African genotypes of wild barley. Photosynthetica, September 2014.

Scientific context

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.

Notes

None

Protein number

Q9STW6 , Q9LTX9

TAIR number

AT4G24280