2 x 50 µl
as08 371-100
624 EUR
Click to
buy
HSP70, heat shock protein 70 cytoplasmic (100µl)
on Gentaurs online shop
Lyophilized
KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 (NP_ 001119156.1), HSC70-2 (NP_195869.1) and HSC70-3 (NP_187555.1) as well as heat shock inducible Hsp70 of Arabidopsis thaliana AT3g12580/T2E22_110 and At1g16030 and AT3g12580/T2E22_110
Rabbit
Polyclonal
Polyclonal
Serum
For reconstitution add 50 µl of sterile water to each tube
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
western blot (WB), immunoprecipitation (IP)
AS08 348 | anti-chloroplastic HSP70AS08 347 | anti-mitochondrial HSP70Plant and algal protein extraction bufferSecondary antibodies
1: 3000 with standard ECL on 5 µg of protein per well (WB), 2-3 µl/protein extract of concentration 3-5 mg/ml
70 kDa
Arabidopsis thaliana, Cucumis sativus, E. teft, Hordeum vulgare, Silene vulgaris, Solanum lycopersicum, Pinus strobus, Zea mays, algae: Desmodesmus subspicatus, phycobiont: Trebouxia TR1 and TR9, Plasmodium falciparum
Chlamydomonas reinhardti, Glycine max, Medicago sativa, Nicotiana bethamiana,Oryza sativa, Phaseolus vulgaris, Physcomitrella patens, Pisum sativum, Populus balsamifera, Solanum lycopersicum, Triticum aestivum, Vitis vinifera, Volvox sp.
no confirmed exceptions from predicted reactivity known in the moment
not available at the moment
Hazlina et al. (2015). Photoinhibition and Development of Stress Proteins in Macroalgae Irradiated with Ultraviolet Radiation. ASM Sci. J., 7(2), 118-128. Guggisberg et al. (2014). A sugar phosphatase regulates the methylerythritol phosphate (MEP) pathway in malaria parasites. Nat Commun. 2014 Jul 24;5:4467. doi: 10.1038/ncomms5467. Liu et al. (2014). Spermidine Enhances Waterlogging Tolerance via Regulation of Antioxidant Defence, Heat Shock Protein Expression and Plasma Membrane H+-ATPase Activity in Zea mays. J. Agronomy and Crop Science, Article first published online: 1 APR 2014, DOI: 10.1111/jac.12058.
Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones
Immunoprecipitation protocol using Agrisera anti-Hsp70 cytosolic antibodies can be found here.
Q9LHA8
AT3G12580
Click to
buy
HSP70, heat shock protein 70 cytoplasmic (100µl)
on Gentaurs online shop
Shock